rabbit polyclonal anti e selectin  (Bioss)

Journal: Biology

Article Title: Canine Angiostrongylus vasorum -Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro

doi: 10.3390/biology10050427

Figure Lengend Snippet: Sequences of canine probes and primers used for qRT-PCR experiments

Article Snippet: Blots were blocked in 3% BSA in TBS (50 mM Tris-Cl, pH 7.6; 150 mM NaCl containing 0.1% Tween (blocking solution); Sigma-Aldrich (Darmstadt, Germany) for 1 h at RT and then incubated overnight at 4 °C in primary antibodies against vinculin (1:1000, #sc-73,614, Santa Cruz), E-selectin (1:500, #TA318934, OriGene, Herford, Germany), P-selectin (1:500, #TA318936, OriGene, Herford, Germany) and VCAM-1 (1:500, #TA502391, OriGene, Herford, Germany) diluted in blocking solution.

Techniques:

Journal: Biology

Article Title: Canine Angiostrongylus vasorum -Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro

doi: 10.3390/biology10050427

Figure Lengend Snippet: Adhesion molecule gene transcription in A. vasorum antigen ( Av Ag)-stimulated canine endothelial cells. CAEC were stimulated with soluble A. vasorum L3 antigen ( Av Ag; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation total RNA was isolated, reverse transcribed and assayed for E-selectin ( A ), P-selectin ( B ), ICAM-1 ( C ) and VCAM-1 ( D ) gene transcription via qPCR. Data are expressed as n -fold of controls (non-stimulated CAEC). TNF-α stimulation was used as positive control.

Article Snippet: Blots were blocked in 3% BSA in TBS (50 mM Tris-Cl, pH 7.6; 150 mM NaCl containing 0.1% Tween (blocking solution); Sigma-Aldrich (Darmstadt, Germany) for 1 h at RT and then incubated overnight at 4 °C in primary antibodies against vinculin (1:1000, #sc-73,614, Santa Cruz), E-selectin (1:500, #TA318934, OriGene, Herford, Germany), P-selectin (1:500, #TA318936, OriGene, Herford, Germany) and VCAM-1 (1:500, #TA502391, OriGene, Herford, Germany) diluted in blocking solution.

Techniques: Incubation, Isolation, Positive Control

Journal: Biology

Article Title: Canine Angiostrongylus vasorum -Induced Early Innate Immune Reactions Based on NETs Formation and Canine Vascular Endothelial Cell Activation In Vitro

doi: 10.3390/biology10050427

Figure Lengend Snippet: Adhesion molecule protein expression in A. vasorum antigen ( Av Ag)-stimulated canine endothelial cells. CAEC cells were stimulated with A. vasorum L3 antigen ( Av Ag; 1 ng/mL) and after 3, 6, 12 and 24 h of incubation, total protein was extracted. Non-stimulated CAEC served as negative controls. The expression of E-selectin ( B ), P-selectin ( C ) and VCAM-1 ( D ) was studied by Western blotting ( A ); representative illustration of one CAEC isolate and densitometric analysis of protein bands. TNF-α was used as positive control of CAEC stimulation. The detection of vinculin was used as loading control for sample normalization. Complete Western Blot acquisitions from E-selectin, P-selectin, VCAM-1 and vinculin is depicted in the .

Article Snippet: Blots were blocked in 3% BSA in TBS (50 mM Tris-Cl, pH 7.6; 150 mM NaCl containing 0.1% Tween (blocking solution); Sigma-Aldrich (Darmstadt, Germany) for 1 h at RT and then incubated overnight at 4 °C in primary antibodies against vinculin (1:1000, #sc-73,614, Santa Cruz), E-selectin (1:500, #TA318934, OriGene, Herford, Germany), P-selectin (1:500, #TA318936, OriGene, Herford, Germany) and VCAM-1 (1:500, #TA502391, OriGene, Herford, Germany) diluted in blocking solution.

Techniques: Expressing, Incubation, Western Blot, Positive Control